人外周血嗜堿性白細胞

CRL-2099 KU812 人外周血嗜堿性白細胞

ATCC® Number: CRL-2099™ Price: $329.00

Designations: KU812

Depositors: K Kishi

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens （human）

Morphology: myeloblast

CRL-2099 KU812 人外周血嗜堿性白細胞

Source: Organ: peripheral blood

Disease: chronic myelogenous leukemia

Cell Type: basophil;

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Isolation: Nigata Japan

Isolation date: August 1, 1981

Antigen Expression: CD3; Homo sapiens

alanyl （membrane） aminopeptidase （ANPEP, CD13）; Homo sapiens

Cytogenetic Analysis: 58, XY, t（9;22）（q34;q11）, i（17q）, +1, +4, +6, +6, +8, +der（9）t（9;22）, + der（9）t（9;22）, +der（11）q（11）, +14, +15, +19, +21, +21, -9 [44220]

Age: 38 years adult

Gender: male

Ethnicity: Japanese

Comments: The KU812 cell line was established from the peripheral blood of a patient in blast crisis of chronic myelogenous leukemia. The cells contain at least one Ph1 （Philadelphia） chromosome. The cell line has some characteristics of basophilic leukocytes （Fc receptors, basophilic granules, histamine production）, and are negative for lymphoid markers.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide （CO2）, 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10（5） viable cells/ml. . Do not allow the cell concentration to exceed 3 x 10（6） cells/ml.

Medium Renewal: every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 20 to 30 hours

Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001

recommended serum:ATCC 30-2020

derivative:ATCC CRL-2100

derivative:ATCC CRL-2101

References: 44220: Kishi K. A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors. Leuk. Res. 9: 381-390, 1985. PubMed: 3858609

44221: Okano H, et al. Hemoglobin synthesis of both adult and fetal types in a human CML cell line. J. Biochem. 104: 162-164, 1988. PubMed: 2460439

44222: Okamura S, et al. Expression of cytokine genes in hematological malignancies. Nippon Ketsueki Gakkai Zasshi 52: 1423-1432, 1989. PubMed: 2698048

61145: Blom T, et al. Phenotypic characterization of KU812, a cell line identified as an immature human basophilic leukocyte. Eur. J. Immunol. 22: 2025-2032, 1992. PubMed: 1639103

CRL-2099 KU812