人骨肉瘤細胞

HTB-94 SW 1353 人骨肉瘤細胞

ATCC® Number: HTB-94™ Price: $338.00

Designations: SW 1353 [SW 1353, SW-1353]

Depositors: A Leibovitz

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens （human）

Morphology: fibroblast

HTB-94 SW 1353 人骨肉瘤細胞

Source: Organ: bone

Disease: chondrosarcoma

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Applications: transfection host （Roche FuGENE® Transfection Reagents）

Antigen Expression: Blood type O-

DNA Profile （STR）: Amelogenin: X

CSF1PO: 12

D13S317: 12,13

D16S539: 11,12

D5S818: 10,11

D7S820: 9,11

THO1: 6,9

TPOX: 8,11

vWA: 16,17

Cytogenetic Analysis: hyperdiploid 47, XX, +7; Except for the trisomic N7 no other chromosome markers are evident

Isoenzymes: AK-1, 1

ES-D, 2

G6PD, B

GLO-I, 2

PGM1, 1

PGM3, 2

Age: 72 years

Gender: female

Ethnicity: Caucasian

Comments: The SW 1353 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1977 from a primary grade II chondrosarcoma of the right humerus obtained from a 72 year old female Caucasian.

The initial culture medium was L-15 containing cortisone and insulin plus 10% fetal bovine serum and antibiotics.

A frozen ampule at passage 12 was received at the ATCC in January, 1982.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subc*tion Ratio: A subc*tion ratio of 1:2 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2008

recommended serum:ATCC 30-2020

HTB-94 SW 1353