人膀胱上皮永生化細胞

CRL-9520 SV-HUC-1 人膀胱上皮永生化細胞

ATCC® Number: CRL-9520™ Price: $417.00

Designations: SV-HUC-1

Depositors: Wisconsin Alumni Res. Fndn.

Biosafety Level: 2 [CELLS CONTAIN ADENOVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens （human）

Morphology: epithelial

CRL-9520 SV-HUC-1 人膀胱上皮永生化細胞

Source: Organ: ureter

Tissue: uroepithelium

Cell Type: epithelial SV40 immortalizedSV40 transformed

Cellular Products: uroepithelial keratins; SV40 T antigen

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Tumorigenic: No

Cytogenetic Analysis: The chromosome number distribution is bimodal with 50% of the cells near diploid （mode = 44 chromosomes） and 50% tetraploid （mode = 88）. Both populations consistently showed seven marker chromosomes: 5p+, del（6）（p11）, 9q+, 11p+, 15q-, 19p+ and Xp+. Most cells lacked a normal chromosome 15. Cells in the tetraploid population did not exactly duplicate the diploid population in that most had a 14q/21 translocation involving two chromosomes 14 and two chromosomes 21, while the diploid cells had normal chromosomes 14 and 21 and a 6q/14q translocation.

Age: 11 years adult

Gender: male

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, add fresh 0.25% Trypsin, 0.03% EDTA solution and allow the flask to sit at room temperature for 20 minutes. Add fresh medium, aspirate and dispense into new flasks.

Subc*tion Ratio: 1:2 to 1:5

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2004

recommended serum:ATCC 30-2020

derivative:ATCC CRL-9519

References: 3992: Reznikoff CA, Christian BJ. Human uroepithelial cell. US Patent 4,980,290 dated Dec 25 1990

CRL-9520 SV-HUC-1