293 人胚腎細胞

CRL-1576 293 人胚腎細胞

ATCC® Number: CRL-1576™

Designations: 12MBr6

Depositors: JL Caputo

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Cercopithecus aethiops

Morphology: epithelial

CRL-1576 293 人胚腎細胞

Source: Organ: lung

Tissue: bronchus

Disease: normal

Cell Type: epithelial

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: July, 1979

Receptors: epidermal growth factor （EGF）

Cytogenetic Analysis: modal number = 62; range = 43 to 64.

The modal chromosome number was found in 78% of the cell population. The rate of polyploidy was 2.4%. Three consistent chromosome markers were identified: M1: del（1）9 ?; M2 del（13） and M3. Chromosome #20 is trisomic and the X chromosome is found in the disomic condition.

Age: 2 to 3 years

Gender: male

Comments: 12MBr6 is an epithelial cell line derived from secondary bronchial tissue of a 2- to 3-year-old normal African green monkey. This line was initiated in June, 1979 at the American Type Culture Collection from tissue explants using penicylinders and a trypsin-versene solution for cell dispersal. These cells contain PAS-positive cytoplasmic inclusions. Epidermal growth factor （EGF） was used to initiate and maintain growth. Post-freeze longevity studies indicate that EGF is an essential component of the culture medium for successful long-term propagation of the cells. Progeny from the 12MBr6 were found to undergo at least 23 additional doublings beyond the frozen state.

Propagation: ATCC complete growth medium: Ham's F-12K Medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 30ng/ml epidermal growth factor and 10% fetal bovine serum

Atmosphere: air, 95%; carbon dioxide （CO2）, 5%

Temperature: 37.0°C

Growth Conditions: The line requires epidermal growth factor as a supplement to the medium.

Subculturing: Protocol: .

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.