RIN-5F大鼠胰腺組織/胰島細胞瘤

RIN-5F大鼠胰腺組織/胰島細胞瘤

Organism    Rattus norvegicus, rat

Tissue    pancreas/islet cell tumor

Cell Type    beta cell

Product Format    frozen

Morphology    epithelial

Culture Properties    adherent

Biosafety Level    1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

RIN-5F大鼠胰腺組織/胰島細胞瘤

Gender    male

Strain    NEDH

Applications

These cells offer models for the study of the biology of pancreatic islet cells, specifically the mechanisms controlling the synthesis, storage and secretion of insulin and somatostatin.

Storage Conditions    liquid nitrogen vapor phase

傳代方法：
收到細胞后，取出培養瓶在倒置顯微鏡下觀察細胞生長情況。
（一）如果細胞未長滿，用75%酒精噴灑整個瓶消毒后放到超菌臺內，嚴格無菌操作，打開細胞培養瓶，吸出培養液，僅留下10ml培養液在瓶內繼續培養。
(二)如果細胞已長滿，即可進行傳代培養。具體步驟如下：
1. 棄去培養液，用PBS（不含鈣，鎂離子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培養瓶中，倒轉放于37度培養箱1-3分鐘預熱，然后又將培養瓶倒轉大約30秒后，在倒置顯微鏡下觀察細胞消化情況，若細胞大部分變圓分散，輕敲幾下培養培養瓶，細胞隨即脫落下來。
3. 加入6-8ml*培養基，吸出，分到新的培養瓶中。1:3~1:6傳代；2~3天1次?！?/span>
。
注意：傳代后一半用我們的培養基，一半用你們的，以免細胞不適應而造
成生長不好。